70-mer oligonucleotides mouse library version 2.0 Search Results


gene synthesis  (Integrated DNA Technologies)
94
Integrated DNA Technologies gene synthesis
Gene Synthesis, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/70-mer+oligonucleotides+mouse+library+version+2%2E0/Gene+Synthesis/custom%40gene-synthesis%4034156286
Average 94 stars, based on 1 article reviews
gene synthesis - by Bioz Stars, 2026-10
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corning hybridization chambers  (Corning Life Sciences)
90
Corning Life Sciences corning hybridization chambers
<t>Cross-hybridization</t> of vRNA to array specific sequences . LR7 cells were either mock-infected or infected with MHV (MOI 10). The cells were incubated with ActD (20 μg/ml) 1 h prior to infection, and maintained in the presence of this drug throughout the experiment. Total RNA was isolated from mock- or MHV-infected cells at 6 h p.i. (A) A representative mRNA amplification plot of a total RNA sample derived from MHV-infected, ActD-treated cells at 6 h p.i. The arrow indicates the marker peak. (B) The scatter plot displays the average expression values from independent dye-swap hybridizations (n = 6) for each gene present on the arrays as described in legend of Fig.2. (C) The Venn diagram shows a comparison between the experiments in the absence or presence of ActD.
Corning Hybridization Chambers, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/70-mer+oligonucleotides+mouse+library+version+2%2E0/hybridization+chamber+corning/pmc02397413-160-33-33
Average 90 stars, based on 1 article reviews
corning hybridization chambers - by Bioz Stars, 2026-10
90/100 stars
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Cross-hybridization of vRNA to array specific sequences . LR7 cells were either mock-infected or infected with MHV (MOI 10). The cells were incubated with ActD (20 μg/ml) 1 h prior to infection, and maintained in the presence of this drug throughout the experiment. Total RNA was isolated from mock- or MHV-infected cells at 6 h p.i. (A) A representative mRNA amplification plot of a total RNA sample derived from MHV-infected, ActD-treated cells at 6 h p.i. The arrow indicates the marker peak. (B) The scatter plot displays the average expression values from independent dye-swap hybridizations (n = 6) for each gene present on the arrays as described in legend of Fig.2. (C) The Venn diagram shows a comparison between the experiments in the absence or presence of ActD.

Journal: BMC Genomics

Article Title: Improved microarray gene expression profiling of virus-infected cells after removal of viral RNA

doi: 10.1186/1471-2164-9-221

Figure Lengend Snippet: Cross-hybridization of vRNA to array specific sequences . LR7 cells were either mock-infected or infected with MHV (MOI 10). The cells were incubated with ActD (20 μg/ml) 1 h prior to infection, and maintained in the presence of this drug throughout the experiment. Total RNA was isolated from mock- or MHV-infected cells at 6 h p.i. (A) A representative mRNA amplification plot of a total RNA sample derived from MHV-infected, ActD-treated cells at 6 h p.i. The arrow indicates the marker peak. (B) The scatter plot displays the average expression values from independent dye-swap hybridizations (n = 6) for each gene present on the arrays as described in legend of Fig.2. (C) The Venn diagram shows a comparison between the experiments in the absence or presence of ActD.

Article Snippet: Mouse slides containing 35,000 spots (32,101 70-mer oligonucleotides, and 2,891 control spots) were hybridized with 1 μg of each alternatively labeled cRNA target at 42°C for 16–20 h using LifterSlips (Erie Scientific) and Corning Hybridization Chambers [ ].

Techniques: Hybridization, Infection, Incubation, Isolation, Amplification, Derivative Assay, Marker, Expressing, Comparison